Restroom? No Go.
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This article is old. Last year…….but you had me thinking and SURFING for over an hour trying to find information on this.
Here’s what I found:

http://www.aa.com/aa/i18nForward.do?p=/customerService/customerCommitment/conditionsOfCarriage.jsp#AircraftInfo

ESSENTIAL NEEDS DURING EXTRAORDINARY DELAYS

In the case of extraordinary events that result in very lengthy onboard delays, American Airlines and American Eagle will make every reasonable effort to ensure that essential needs of food (snack bar such as Nutri-Grain®), water, restroom facilities, and basic medical assistance are met. We are not responsible for any special, incidental or consequential damages if we do not meet this commitment.

The Disability ACT requires:
Accessibility of facilities

New aircraft with 30 or more seats must have movable aisle armrests on half the aisle seats in the aircraft. “New aircraft” requirements apply to planes ordered after April 5, 1990 or delivered after April 5, 1992. No retrofit is required, although compliance with on-board wheelchair requirements (see below) became mandatory on April 5, 1992 regardless of the plane’s age. As older planes are refurbished, required accessibility features (e.g., movable armrests) must be added.

New widebody (twin-aisle) aircraft must have accessible lavatories.

New aircraft with 100 or more seats must have priority space for storing a passenger’s folding wheelchair in the cabin.

Aircraft with more than 60 seats and an accessible lavatory must have an on-board wheelchair, regardless of when the aircraft was ordered or delivered. For flights on aircraft with more than 60 seats that do not have an accessible lavatory, carriers must place an on-board wheelchair on the flight if a passenger with a disability gives the airline 48 hours’ notice that he or she can use an inaccessible lavatory but needs an on-board wheelchair to reach the lavatory

THE AIRCRAFT THAT OPERATES THAT ROUTE IS AN ER3 which HOLDS 37 SEATS and IS A ONE HOUR FLIGHT.

http://www.aa.com/aa/seatmap/viewSeatsSubmit.do?flightNumber=4670&departureMonth=8&departureDay=6&originAirport=RDU&destinationAirport=DCA&cabin=Coach&bookingPathStateId=1217554703323-944&returnUrl=%2Freservation%2FredisplayFlightSearchResults.do

I’ve actually flew on a small aircraft WITHOUT any lavatory. BOS to Cape Code.
I could swear that you HAVE TO HAVE a working lavatory on a MAJOR commercial JET. NO GO ITEM.

was interesting and fun, searching your post. =)

Restroom? No Go.

Half an hour before a flight from Raleigh-Durham to Reagan National Airport, an American Airlines gate agent announced that the only restroom on the small plane was out of order and would be unavailable for the duration of the hour-long flight .

Passenger Amy Grulke of Fairfax asked CoGo: Can it be legal for a plane to depart with no working restroom?

“We have no rules regarding restrooms,” said U.S. Department of Transportation spokesman Bill Mosley. He suggested that CoGo contact the Environmental Protection Agency.

The EPA does set water-quality standards for aircraft, spokesman Dale Kemery said. He promised to investigate whether the agency has any further involvement. Soon he called back. “We don’t do toilets,” he said, and suggested contacting the Federal Aviation Administration.

Not us , said FAA spokeswoman Alison Duquette, who said that if there were a rule, it would be an EPA rule.

CoGo’s advice: Hold it.

Memo to Airline passengers: Please try and treat this MULTI MILLION DOLLAR VESSEL with respect. Is there some reason you had to paint your nails inflight and get polish all over the tray table? Let your children color, and do the same?
Pull out power ports from the armrests? Put your dirty feet on the wall? Throw a dirty diaper on the floor? Crack the lavatory mirror? Stain the seats with playdough, ink pens and, yes, I’ve even seen defecation stains. Graffiti, everywhere. After passengers deplane, I look around and cant believe HUMANS were actually onboard.